Effects of the Demethylating Agent, 5-Azacytidine, on Expression of the Kallikrein-Kinin Genes in Carcinoma Cells of the Lung and Pleura
Figure 3
Basal kallikrein-kinin mRNA and protein expression in JU77, NO36, and LO68 malignant mesothelioma cells and in nonmalignant Met5A mesothelial cells. (a) Fold differences in mRNA expression for the tissue kallikrein (KLK1), plasma kallikrein (KLKB1), kinin B1 receptor (BDKRB1), and kinin B2 receptor (BDKRB2) genes in JU77, NO36, and LO68 cells, relative to that in Met5A cells (arbitrarily set at 1). (b) Mean cytoplasmic expression of tissue kallikrein (TK), plasma kallikrein (PK), kinin B1 receptor (B1R), kinin B2 receptor (B2R), and high molecular weight kininogen (HK) in Met5A, JU77, NO36, and LO68 cells, as assessed by immunofluorescence labelling and image analysis.
