Effects of the Demethylating Agent, 5-Azacytidine, on Expression of the Kallikrein-Kinin Genes in Carcinoma Cells of the Lung and Pleura
Figure 2
Effect of 5-azacytidine (5-AZA) on kallikrein-kinin mRNA expression in H2126 lung adenocarcinoma, H520 lung squamous cell carcinoma, and nonmalignant BEAS-2B lung epithelial cells. (a) Fold changes in mRNA expression for the tissue kallikrein (KLK1), plasma kallikrein (KLKB1), kinin B1 receptor (BDKRB1), and kinin B2 receptor (BDKRB2) genes, relative to vehicle control (arbitrarily set at 1), in BEAS-2B cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA. (b) Fold changes in mRNA expression for the KLK1, KLKB1, BDKRB1, BDKRB2, and high molecular weight kininogen (KNG1) genes, relative to vehicle control (arbitrarily set at 1), in H2126 cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA. (c) Fold changes in mRNA expression for the KLK1, KLKB1, BDKRB1, BDKRB2, and KNG1 genes, relative to vehicle control (arbitrarily set at 1), in H520 cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA.
