Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

Table 1

Gene-specific primers used in quantitative real-time PCR.

Gene	Forward	Reserve	Gene accession number
GAPDH	5′-CATGAGAAGTATGACAACAGCCT-3′	5′-AGTCCTTCCACGATACCAAAGT-3′	NM_001256799.2
CD31	5′-GAGTCCTGCTGACCCTTCTG-3′	5′-TCAGGTTCTTCCCATTTTGC-3′	NM_000442.4
Vimentin	5′-GAAATTGCAGGAGGAGATGC-3′	5′-ATTCCACTTTGCGTTCAAGG-3′;	NM_003380.3
α-SMA	5′-CTGAGCGTGGCTATTCCTTC-3′	5′-AGAAGAGGAAGCAGCAGTGG-3′	NM_001141945.2
Collagen I	5′-ACCTGGTCAAACTGGTCCTG-3′	5′-CCTGTGGTCCAACAACTCCT-3′	NM_000088.3
Collagen III	5′-GATCAGGCCAGTGGAAATGT-3′	5′-GTGTGTTTCGTGCAACCATC-3′	NM_000090.3
CTGF	5′-GTTCCAAGACCTGTGGGATG-3′	5′-TCTCTTCCAGGTCAGCTTCG-3′	NM_001901.2
Fn	5′-CAAACTCCGTCACCCTCAGT-3′	5′-GGTGCCAGTGGTTTCTTGTT-3′	NM_001306129.1

CTGF: connective tissue growth factor; Fn: fibronectin.