Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells
Figure 4
PPARγ binds to the PPRE within the 5′-flanking region of LPP1. HUVECs were coinfected with Ad-WT-PPARγ and Ad-tTA for 48 h. ChIP assays were performed with the antibodies against PPARγ or IgG as a negative control. Immunoprecipitated DNA fragments were detected by qPCR with the use of specific primers spanning the DNA segments containing the predicted PPREs of LPP1 promoter. Data were mean ± SEM of three independent experiments. * versus control.
