The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver
Figure 2
HNF4α is a master gene for the hepatic expression of Thb. (a) Expression of hepatic Thb in HNF4α null (Hnf4 loxP/loxPAlfp.cre; loxP/loxP) and control (Hnf4 loxP/+Alfp.cre; loxP/+) mouse embryos was determined by semiquantitative RT-PCR analysis ( per group). Hprt mRNA livers were evaluated and used as internal standard of loading. Tha: thiolase A; Thb: thiolase B; Hnf4α: Hepatocyte Nuclear Factor-4 alpha; Hprt: Hypoxanthine-guanine PhosphoRibosyl Transferase. (b) Mouse Hepa 1.6 hepatoma cells were transfected with expression vectors for WT HNF4α or dominant negative form of HNF4α (DN HNF4). Expression of Thb, Tha, and apolipoprotein AII (ApoA-II) was determined by quantitative RT-PCR. Crude results were standardized against 36B4 mRNA levels. Levels of gene expression in Hepa 1.6 cells transfected with empty pcDNA3.1 vector serve as reference point and are given the arbitrary value of 1.0. Values are mean of three independent experiments ±SEM. Apolipoprotein AII (ApoAII) gene expression was used as a positive control of experiment. Significantly different compared to control (transfection with empty pcDNA3.1 vector) with * and ** by one-way ANOVA test. n.s.: no statistically different compared to control.
