Peroxisome Proliferator-Activated Receptor -β/δ, -γ Agonists and Resveratrol Modulate Hypoxia Induced Changes in Nuclear Receptor Activators of Muscle Oxidative Metabolism

Figure 6

The effect of PPAR-γ agonist (Rosiglitazone, 10   μM) treatment for 24 hours, following 7 days of differentiation under relative oxygen regimes, 21%, 5%, and 1%. Fold expression of SIRT1 (a), PGC-1α (b), PPAR-α (c), RXR-α (d), CPT-1b (e) mRNA, and SIRT1 protein (f) with (+) and without (−) Rosiglitazone. Values represent the mean of 3-4 experiments. Meanvalues±SEM. The effect of Rosiglitazone within each oxygen regime was evaluated by Students 𝑡-test within oxygen regime, *𝑃<.05, **𝑃<.01.

(a) SIRT1

(b) PGC-1α

(c) PPAR-α

(d) RXR-α

(e) CPT-1b

(f) SIRT1