PPAR Action in Human Placental Development and Pregnancy and Its Complications
Figure 3
Immunoblot of JEG-3 cells treated with pooled sera (10%) from nonpregnant
(NP), pregnant (P), and preeclamptic (PE) women. Cell lysates were
analyzed using a specific mouse anti-human PPARγ monoclonal antibody. Equal
amounts of protein (50 μg) were loaded into each lane. Factors in pregnant
serum up-regulate JEG-3 PPARγ expression. A decrease in PPARγ protein was
observed in cells exposed to PE sera (PE) compared to sera from normal
pregnant women (P).