Therapeutic Role of Protein Tyrosine Phosphatase 1B in Parkinson’s Disease via Antineuroinflammation and Neuroprotection In Vitro and In Vivo

Figure 3

Effect of suramin on 6-OHDA-induced downregulation of phospho-extracellular signal-regulated kinases (p-ERK), phospho-cAMP response element-binding protein (CREB), and brain-derived neurotrophic factor (BDNF) in SH-SY5Y cells. (a) SH-SY5Y cells were pretreated with 10 μM suramin for 1 h and then challenged with 20 μM 6-OHDA for 1 h; western blotting for p-ERK and p-CREB of the control, 6-OHDA, 6-OHDA plus suramin, and suramin alone groups is shown. SH-SY5Y cells were pretreated with 10 μM suramin for 1 h and then challenged with 20 μM 6-OHDA for 8 h; western blotting for BDNF of the control, 6-OHDA, 6-OHDA plus suramin, and suramin alone groups is shown. β-Actin was used as an internal control. (b) The quantification results of p-ERK relative density are shown. (c) The quantification results of p-CREB relative density are shown. (d) The quantification results of p-ERK relative density are shown. Data are presented as mean ± SEM, and each value contains three replicates and three samples. Significantly different from the control group; significantly different from the 6-OHDA group.

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