Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

Figure 2

4,5-diCQA decreases DU-145 cell proliferation after withdrawal of treatment. Clonogenic assays were performed to determine the long-term effects of 4,5-diCQA on DU-145 cells. The treated cells were left to proliferate for 9 days in drug-free medium. (a) Representative image of the fixed and stained clonogenic assays showing reduced colony forming efficiency of the treated cells on the bottom row as compared to control on top row. (b) Colonies were counted and mean values of four trials standard deviations are represented. The treated group had significantly less colonies than the control (p-value = 5.64E-06).

(a)

(b)