Research Article
Mitochondrial Oxidative Stress Impairs Energy Metabolism and Reduces Stress Resistance and Longevity of C. elegans
Table 1
Oligonucleotide primer sequences and product sizes for quantitative real-time PCR. Concentration was 0.1 μM for all primers.
| Primer | Sequence | Product size (bp) |
| Aak-2 | 5-tgcttcaccatatgctctgc-3 5-gtggatcatctcccagcaat-3 | 219 |
| Ama-1 | 5-ccaggaacttcggctcagta-3 5-tgtatgatggtgaagctggcg-3 | 85 |
| Act-2 | 5-cccactcaatccaaaggcta-3 5-gggactgtgtgggraacacc-3 | 168 |
| Atfs-1 | 5-tcggcgatcgatcagctaac-3 5-agaatcagttcttggattagggga-3 | 75 |
| Atp-2 | 5-tccaagtcgctgaggtgttc-3 5-aggtggtcgagttctcctga-3 | 151 |
| Daf-16 | 5-tcctcattcactcccgattc-3 5-ccggtgtattcatgaacgtg-3 | 175 |
| Sir-2.1 | 5-tggctgacgattcgatggat-3 5-atgagcagaaatcgcgacac-3 | 179 |
| Skn-1 | 5-acagggtggaaaaagcaagg-3 5-caggccaaacgccaatgac-3 | 246 |
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bp: base pairs; Conc: concentration.
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