Mitochondrial Oxidative Stress Impairs Energy Metabolism and Reduces Stress Resistance and Longevity of C. elegans

Figure 7

(a) Mitochondrial membrane potential (ΔΨm) over 130 minutes assessed by fluorescent dye rhodamine 123 (Rh123). Short circuit of ΔΨm was achieved by the addition of FCCP after 30, 60, and 120 minutes. (b) ΔΨm (MMP), assessed by mitochondria-dependent increase of Rh123 fluorescence in percentage, after PQ treatment (5 mM) for 48 h, detected with a ClarioStar Plate Reader (BMG, Ortenberg, Germany). (c) Respiration of isolated mitochondria from C. elegans normalized to (d) citrate synthase activity in international units IU/(mg/mL protein). Activity of respiration complexes was measured using an O2k Oxygraph (Oroboros, Innsbruck, Austria). Addition of substances into the Oxygraph’s chambers is indicated with a plus sign (+). ; Student’s -test; , , , and .

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