Phosphocreatine Improves Cardiac Dysfunction by Normalizing Mitochondrial Respiratory Function through JAK2/STAT3 Signaling Pathway In Vivo and In Vitro

Figure 7

PCr enhances mitochondrial respiration. (a) Effects of PCr on mitochondrial respiration in intact H9C2 cells. Agent respiratory explore different avenues regarding control H9C2 cells. (b) MGO-incited H9C2 cells. (c) PCr (20 mM) pretreated with MGO (1 mM) invigorated cells. (d) Quantification for all groups in intact H9C2 cells. The respirometry convention for intact cells included the ROUTINE state which was estimated when a steady oxygen transition had been gotten following expansion of cells into the test chamber. Uncoupled respiration in the LEAK state was estimated when a consistent oxygen motion had been accomplished after the expansion of oligomycin (2.5 μM), FCCP (0.5 μM steps), rotenone (0.5 μM), and antimycin A (2.5 μM). (e) Effect of PCr on permeabilized H9C2 cells in control. (f) MGO-induced H9C2 cells. (g) PCr (20 mM) pretreated with MGO-stimulated cells. (h) Quantification for all the groups in permeabilized H9C2 cells. The following are added: digitonin (8.1 μM, 10 μg/10⁶ cells); P: pyruvate (5 mM); G: glutamate (10 mM); M: malate (2 mM); ADP (5 mM); S: succinate (10 mM); FCCP (0.5 μM steps); rotenone (0.5 μM); antimycin A (2.5 μM). Data are presented as the ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)