Phosphocreatine Improves Cardiac Dysfunction by Normalizing Mitochondrial Respiratory Function through JAK2/STAT3 Signaling Pathway In Vivo and In Vitro

Figure 1

Mitochondrial respiration in heart tissue homogenate and isolated heart mitochondria of rats. (a) Healthy heart rat tissue homogenate and the mitochondrial respiration were detected using Oxygraph-2k. Red lines indicate the oxygen consumption rate in response to sequential loading of mitochondrial effectors (indicated by arrows above the graphs). (b) STZ group. (c) The treatment group with PCr (50 mM). (d) Quantitative examinations of oxygen utilization rate in light of effectors. Correlations were performed utilizing data lab programming. (e) Isolated heart mitochondria from healthy rats. (f) STZ group. (g) The treatment group with PCr (50 mM). (h) Quantitative examinations of oxygen utilization rate in light of effectors. The following are added: P: pyruvate (5 mM); G: glutamate (10 mM); M: malate (2 mM); ADP: adenosine diphosphate (5 mM); Cyt C: cytochrome c (10 μM); S: succinate (10 mM); FCCP (0.5 μM steps); rotenone (0.5 μM); antimycin A (2.5 μM). Data are displayed as the ( per group), and comparisons were performed using DatLab software.

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