Research Article

Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway

Figure 7

Glycine protected PC12 cells against CoCl2-induced injury via regulation of mitochondria-mediated autophagy. (a) Protein expression level of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in PC12 cells of each group. (b) Analyses of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group. (c) After treatment of CsA, protein expression levels of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in PC12 cells of four groups were determined by Western blotting. (d) Analysis of all protein expressions. (e) Cell viability of four groups was detected by CCK8. (f) Apoptotic cells from each group were measured by TUNEL and DAPI staining. (g) Mitochondrial ROS generations were detected by immunofluorescence of MitoSOX and DAPI staining. (h) Mitochondrial membrane potential was detected in each group by TMRE and Hoechst staining. (i) The Mfn-2, protein of mitochondrial outer membrane, was used to measure function of mitochondria. , , and . .
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