Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway

Figure 2

Glycine protected against hypoxic-ischemic injury in the brains via attenuation of mitochondrial-mediated autophagy. (a) Protein expression level of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I on the cortex from ipsilateral sides of the sham group, HIE group, and HIE with administration of the glycine group (of GAPDH). (b) Analyses of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group. (c) Protein expression level of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I on the hippocampus from ipsilateral sides of each group (of GAPDH). (e) Analyses of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group. (e) Protein expression level of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group from the cortex. (f) Analyses of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group. (g) Protein expression level of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group from the hippocampus. (h) Analyses of Mfn-2, p62, parkin, PINK1, Bnip3, LC3 II, and LC3 I in each group. (i) HE stainings of the cortex of four groups. (j) HE stainings of the hippocampus among four groups. (k) Nissl stainings in the cortex from four groups and neuron numbers were analyzed. . (l) Nissl stainings in the hippocampus from four groups and neuron numbers were analyzed. , , and . .

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