Vanillic Acid Restores Coenzyme Q Biosynthesis and ATP Production in Human Cells Lacking COQ6

Figure 2

(a) HPLC electrochemical detection analyses (with precolumn electrode in oxidizing mode) of lipid extracts from wild-type (WT) and COQ6∆25 cells (0.2 mg protein) with UQ₈ used as standard. UQ₁₀ and 4-HP₁₀ have different retention times as shown by the dotted lines. (b, c) Single-ion monitoring ((b) 806.5, (c) 808.5) in HPLC mass spectrometry analyses of lipid extracts from WT and COQ6∆25 cells (0.2 μg protein) with the precolumn electrode set in oxidizing mode (ox) or reducing mode (red). The chemical structures of (b) 3-decaprenyl-1,4-benzoquinone and (c) 3-decaprenyl-1,4-benzoquinol are shown.

(a)

(b)

(c)