Occupational Exposure to Pesticides in Tobacco Fields: The Integrated Evaluation of Nutritional Intake and Susceptibility on Genomic and Epigenetic Instability
Table 5
Micronucleus (MN), nucleoplasmic bridge (NPB), nuclear buds (NBUD), and binucleated (BN) cell frequencies, telomere length (bp), and % of global DNA methylation for nonexposed and exposed groups according to MTHFR and TERT polymorphisms. Data presented as error (SE).
Parameters
MTHFR (rs1801133)
a
TERT (rs2736100)
b
CC ()
CT/TT ()
GG ()
GT/TT ()
CBMN
Micronucleusc
Nonexposed
3.2 ± 0.7 (16)
2.6 ± 0.4 (23)
0.427
2.6 ± 0.5 (11)
2.9 ± 0.5 (28)
0.766
Exposed
5.0 ± 0.8 (10)
6.2 ± 0.6 (28)
0.307
7.6 ± 1.4 (7)
4.9 ± 0.5 (32)
0.029
Nucleoplasmic bridgec
Nonexposed
2.2 ± 0.4 (17)
2.7 ± 0.7 (23)
0.555
2.2 ± 0.6 (11)
2.7 ± 0.5 (28)
0.589
Exposed
6.6 ± 0.7 (10)
4.7 ± 0.5 (28)
0.034
5.0 ± 1.0 (7)
5.5 ± 0.5 (32)
0.641
NBUDc
Nonexposed
1.5 ± 0.4 (17)
2.2 ± 0.5 (23)
0.439
2.0 ± 0.5 (11)
2.0 ± 0.6 (28)
0.937
Exposed
4.7 ± 0.4 (10)
3.0 ± 0.3 (28)
0.013
3.6 ± 0.6 (7)
3.1 ± 0.3 (32)
0.535
Binucleated cellsd
Nonexposed
56.3 ± 7.0 (17)
38.1 ± 3.9 (20)
0.046
49.5 ± 7.1 (12)
44.9 ± 4.9 (28)
0.616
Exposed
106.7 ± 18.6 (10)
121.8 ± 9.6 (28)
0.441
135.3 ± 15.0 (7)
115.1 ± 9.7 (31)
0.359
Telomere length
Nonexposed
4266 ± 150.7 (16)
5497 ± 468.4 (23)
0.053
5824 ± 713.9 (11)
4392 ± 125.1 (28)
0.049
Exposed
3918 ± 206.9 (10)
4406 ± 200.3 (28)
0.164
4504 ± 765.9 (7)
4161 ± 122.4 (31)
0.656
Global DNA methylation
Nonexposed
3.779 ± 0.34 (16)
3.821 ± 0.26 (23)
0.922
3.659 ± 0.32 (10)
3.862 ± 0.26 (25)
0.662
Exposed
2.784 ± 0.24 (10)
3.335 ± 0.14 (28)
0.049
2.596 ± 0.26 (5)
3.075 ± 0.12 (30)
0.161
a value between the CC genotype and the combination CT/TT within groups; b value between the GG genotype and the combination GT/TT within groups; cevaluated in 1000 BN cells per individual; devaluated in 500 cells per individual.