Oxidative Medicine and Cellular Longevity

Research Article

Effect of Neonatal Exposure to Poly(Ethylene Glycol)-block-Poly(Lactic Acid) Nanoparticles on Oxidative State in Infantile and Adult Female Rats

Table 2

Effects of neonatal PEG-b-PLA NP exposure on oxidative status of infantile and adult female Wistar rats.
ParameterControlPEG20PEG40
Infantile rats   
TEAC (mmol trolox/l)3.046 (2.882–3.313)3.07 (2.952–3.39)2.124∗∗## (1.826–2.870)
Protein carbonyls (nmol/mg protein)1.772 (1.43–2.10)2.305 (2.00–2.356)1.931 (1.844–2.059)
Lipoperoxides (nmol/ml)184.861 (154.960–285.039)222.400 (170.020–251.429)254.702 (189.663–275.109)
CAT (μkat/g Hb)0.724 (0.565–0.896)0.818∗ (0.768–1.034)1.111∗ (0.821–1.168)
GPx (nU/ml/mg Hb)36.585 (31.610–42.125)43.506 (32.127–49.125)47.829∗∗∗ (45.864–54.859)
SOD (U/mg Hb)8.347 (6.508–10.736)11.417 (9.837–12.721)12.985∗∗∗ (11.345–14.144)
Adult rats   
TEAC (mmol/l)2.842 (2.758–3.004)2.586 (2.518–2.890)2.951 (2.9525–2.955)
Protein carbonyls (nmol/mg protein)1.843 (1.799–2.310)1.692∗ (1.649–1.722)1.863# (1.859–2.087)
Lipoperoxides (nmol/ml)44.306++ (36.230–55.655)43.541 (34.920–72.788)46.488 (32.956–53.909)
CAT (μkat/g Hb)56.538+++ (56.078–57.434)0.592 (0.493–0.633)0.538 (0.523–0.657)
GPx (nU/ml/mg Hb)56.538+++ (56.078–57.434)53.204 (46.612–57.434)41.122∗ (40.200–50.426)
SOD (U/mg Hb)12.479+ (10.703–14.847)8.738∗ (7.340–10.202)6.325∗ (4.944–7.938)
TEAC: trolox equivalent antioxidant capacity; CAT: catalase activity; GPx: glutathione peroxidase; SOD: superoxide dismutase activity; PEG20: animals treated with PEG-b-PLA NPs (20 mg/kg body weight); PEG40: animals treated with PEG-b- PLA NPs (40 mg/kg body weight). Data are presented as the median with interquartile range (Q1-Q3, 25–75%). Statistical analysis was performed using Stats Direct 3 Statistical software and Mann–Whitney U test. PEG 20 or PEG40 versus control; PEG 20 or PEG40 versus control; PEG 20 or PEG40 versus control; # PEG40 versus PEG20; ## PEG40 versus PEG20; control-adult rats versus control-infantile rats; control-adult rats versus control-infantile rats; and control-adult rats versus control-infantile rats.