NRF2 Plays a Critical Role in Both Self and EGCG Protection against Diabetic Testicular Damage

Figure 6

EGCG enhanced testicular NRF2 expression and function. Testicular (a) t-NRF2 and (b) n-NRF2 protein were determined by Western blot in all the mice. (c) The ratio of n-NRF2 to t-NRF2 was calculated to indicate NRF2 nuclear translocation. To evaluate NRF2 function, the expression of Nqo1 and Ho1 were further determined, by measuring Nqo1 (d) mRNA and (e) protein levels, as well as Ho1 (f) mRNA and (g) protein levels. t-NRF2 protein and the expression of Nqo1 and Ho1 were normalized to GAPDH. n-NRF2 was normalized to Histone H3. Data were normalized as fold variation to WT Ctrl and were presented as means ± SD (). versus WT Ctrl; ^† versus WT DM. t-NRF2: total NRF2; n-NRF2: nuclear NRF2; Nqo1: NAD(P)H dehydrogenase quinone 1; Ho1: heme oxygenase-1; other abbreviations are the same as those in Figure 1.

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