Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin

Figure 7

Periodic oxidative redox challenges decelerated keratinocytes motility and migration. Scratch assays were performed to monitor artificial wound healing over 24 h. Representative images are shown for controls and repeatedly (1–8 weeks) plasma-treated cells (a). There was a significant decrease in cell motility following plasma induced redox stress (b). Compared to control cells (ctrl) plasma-treated cells (w2) displayed a notable enlargement in size as seen after staining cell margins for ZO-1 (c). Mitotracker red fluorescence (MFI), which is a marker for total mitochondrial content, increased after chronic stress and was measured using flow cytometry (d). Data are presented as mean + SD of two to four experiments and statistical comparison was done using ANOVA with Dunnett correction for multiple comparison to untreated, normalized control (b), or Student’s -test (d) (, , ).

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