Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin

Figure 6

Western blot analysis showed a differential expression of several redox-related proteins. Western blot analysis confirmed the RNA expression profiles of β-actin ((a)(I), no change) and of the antioxidative enzymes NQO1 ((a)(II)) and PRDX2 ((a)(III)). Puma and p21 ((b)(I), (b)(II)) were upregulated after chronic but not acute oxidative challenge whereas keratin 1 was downregulated ((b)(III)). The total amount of H2AX protein was twofold downregulated after periodic oxidative challenge in contrast to acute stress ((c)(I)) while its phosphorylated form γ-H2AX was slightly upregulated in response to oxidative stress ((c)(II)). The ratio of p-H2AX to the total protein amount of H2AX was significantly enhanced after chronic oxidative challenge ((c)(III)). The total amount ((d)(I)) and the phosphorylated form ((d)(II)) of the stress-related protein HSP27 was significantly enhanced after repeated plasma treatment. The ratio of p-HSP27 to total protein amount of HSP27 was significantly decreased ((d)(III)). Representative western blot images are shown. Data in diagrams are presented as mean + SD of two independent experiments. Statistical analysis was performed using one-way ANOVA with Dunnett corrections for multiple testing to untreated, normalized control (, , ).

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