Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin

Figure 5

Apoptosis-related gene expression in response to periodic redox challenge. One hundred and fifteen apoptosis-related genes were associated with frequent redox challenges whereas 17 and 31 genes belonged to negative or positive regulation apoptosis, respectively (a). Downstream targets of p53 signaling pathway included BCL2 and BBC3 which represent the proapoptotic p53 pathway and GADD45 that is involved in DNA repair. All of them were mainly upregulated. CDKN1A plays a role in cell cycle control and senescence and was slightly downregulated (b). Expression of stress-related heat shock proteins was partially enhanced as well (c). Data are presented as mean + SD of two analyses (b, c). Statistical analysis was done using one-way ANOVA with Dunnett corrections for multiple comparisons to untreated, normalized control (, , ).

(a)

(b)

(c)