Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin

Figure 1

Exposure to plasma-treated medium evoked acute oxidative stress but was not cytotoxic. HaCaT keratinocytes were loaded with CM-H₂DCF-DA and exposed to plasma-treated medium (60 s). Cells were subsequently trypsinized and fluorescence was measured using flow cytometry (a). Compared to control cells, intracellular mean fluorescence intensity (MFI) of DCF was significantly increased in treated keratinocytes (b). To assess viability, keratinocytes were exposed to plasma-treated medium and incubated for 18 h. Cells were stained for active caspase 3 and analyzed by flow cytometry (c). The number of apoptotic cells was significantly increased in etoposide control but not in cells exposed to plasma-treated medium (d). Data are presented as one representative (a, c) or mean + SD (b, d) of two independent experiments. Statistical comparison was done using Student’s -test (b) or one-way ANOVA with Dunnett corrections for multiple comparison to untreated control (d) (, ).

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