Impact of Antioxidants on Cardiolipin Oxidation in Liposomes: Why Mitochondrial Cardiolipin Serves as an Apoptotic Signal?

Figure 5

Prevention of the MeO-AMVN-induced oxidation in CL liposomes by 1 μM Q₁₀H₂. Conditions: pH-buffer A (see Section 2), 100 μM CL, 50 μM MeO-AMVN, and 40°C. (a, b) Spectral changes of the liposome suspension over the whole time range (a) and during the induction phase (b). (c) Kinetics of absorbance changes at 234 nm (accumulation of conjugated dienes) and at 281 nm (“oxidized minus reduced” absorption difference for Q₁₀, cf with Figure 4(b)) show that lipid peroxidation did not start before the antioxidant Q₁₀H₂ was exhausted.