Research Article
Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer
Table 4
supF mutations in a supF forward mutation assay using the pMY189 plasmid containing 8-hydroxyguanine (8OHG) at position 159 of supF in H1299 human cell lines inducibly expressing MUTYH protein.
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Empty vector-transposed H1299 human cancer cell line and H1299 cells inducibly expressing type 2 MUTYH protein of WT, p.Arg81Trp, or p.Asp208Asn were used. bThe shuttle plasmid pMY189, containing 8-hydroxyguanine (8OHG) at nucleotide position 159 of supF, or a wild-type (WT) pMY189 plasmid was used. cThe value for the difference in the proportion between cells transfected with an 8OHG-containing pMY189 plasmid was <0.0001 (Fisher exact test). dThe value for the difference in the proportion between cells transfected with an 8OHG-containing pMY189 plasmid was <0.001 (Fisher exact test). |