Supplemental Cellular Protection by a Carotenoid Extends Lifespan via Ins/IGF-1 Signaling in Caenorhabditis elegans

Table 2

Effect of AX on DAF-16 localization into the nucleus in several age-related mutants.

Strain (condition)	Age (days)	Number of DAF-16-translocated nuclei (/unit area)
N2 (control)	4	N.D.
N2 (1 mM AX)	4	N.D.
N2 (control)	14	3.4 ± 0.5
N2 (1 mM AX)	14	12.0 ± 2.0*
age-1 (control)	4	12.6 ± 1.1
age-1 (1 mM AX)	4	13.6 ± 3.2
age-1 (control)	14	14.2 ± 2.3
age-1 (1 mM AX)	14	13.6 ± 1.3

DAF-16-translocated nuclei were counted mainly in the epithelia, musculature, and intestine. N.D. indicated not detected. Results about the number of DAF-16-translocated nuclei are indicated as means ± SD from more than five independent transgenic animals. 𝑃 values by 𝑡-test with an asterisk (controls versus AX-treated condition) significantly differ as follow; *𝑃<0.001.