Supplemental Cellular Protection by a Carotenoid Extends Lifespan via Ins/IGF-1 Signaling in Caenorhabditis elegans

Figure 2

mRNA expression levels of sod and ctl genes in wild-type N2, age-1(hx546), and daf-16(mgDf50) animals using RT-PCR. Each mRNA of 14-day-old animals was prepared and analyzed. Panels indicate the quantitative data obtained using the luminescent image analyzer and AU in the panels indicates arbitrary unit. Data are means ± SD of three or more independent experiments. Left-hand open column and right-hand shaded column for each strain indicate values without and with 1 mM AX-exposure, respectively. Asterisk indicates significant difference during the values without and with AX exposure. 𝑃 values, which were calculated using a two-tailed 𝑡-test for paired samples with unequal variances, are ^∗𝑃<0.05 and ^∗∗𝑃<0.005.

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