Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos
Figure 6
Fluorescence immunocytochemistry of Ac-H3 in oocytes and embryos. (a) Expression of Ac-H3 in 2-cell, 4-cell, and 8-cell embryos from IVM and control groups. Cells were immunostained with the Ac-H3 antibody. Each sample was counterstained with DAPI (blue) to visualize the DNA. Specific rabbit polyclonal Ac-H3 was detected by fluorescein-conjugated antirabbit secondary antibodies (green, FITC-labeled). Bar represents . (b) The gray value of GCN5 in oocytes and embryos.
