TY - JOUR A2 - Jiang, Shi-Wen AU - Barekati, Zeinab AU - Radpour, Ramin AU - Kohler, Corina AU - Zhong, Xiao Yan PY - 2010 DA - 2010/07/27 TI - Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes SP - 870865 VL - 2010 AB - DNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers. A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast cancer). However, still clinical use of them is very limited because of lack of specificity and sensitivity for diagnostic test. This highlights the critical need for specific primer and probe design to avoid false-positive detection of methylation profiling. The guideline and online web tools that are introduced in this paper might help to perform a successful experiment and to develop specific diagnosis biomarkers by designing right primer pair and probe prior to experimental step. SN - 1687-9589 UR - https://doi.org/10.1155/2010/870865 DO - 10.1155/2010/870865 JF - Obstetrics and Gynecology International PB - Hindawi Publishing Corporation KW - ER -