Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model

Figure 2

PanNaV, NaV1.1, and NaV1.2 expression in the PFC of the BTBR mouse model. (a, b) High-resolution confocal images where the red channel represents confocal images of PanNaV, the blue channel represents FGF14, and the green channel represents NeuN immunofluorescence in the PFC of C57BL/6J and BTBR mice. (c) Western blot analysis for PanNaV, NaV1.1, and NaV1.2 was performed on PFC homogenate and quantified. (d) The upregulation of PanNaV, NaV1.1, and NaV1.2. The protein expression was normalized with β-actin. Data represent ; statistical differences were assessed using Student’s -test (, -5 mice per group). Scale bars represent 20 μm in (b) and 10 μm in zoom images in (b).