Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

Figure 5

Representative micrographs of estimated number of regenerated myelinated axons of the sciatic nerve, 4, 8, and 12 weeks, following P2 sciatic nerve transection and repair. Note smaller fibers and counting in both coaptation groups compared with the contralateral nerve. Axotomy only group did not show any regenerated fiber. (a, b) Ipsilateral side, 4 weeks after lesion, groups AX+FS and AX+CFS, respectively. (c) Contralateral side, 4 weeks after lesion. (d, e) Ipsilateral side, 8 weeks after lesion, groups AX+FS and AX+CFS, respectively. (f) Contralateral side, 8 weeks after lesion. (g, h) Ipsilateral side, 12 weeks after lesion, groups AX+FS and AX+CFS, respectively. (i) Contralateral side, 12 weeks after lesion. Scale bar = 10 μm. (j) Estimated number of regenerated myelinated fibers following P2 sciatic nerve transection and repair. Mean SE. AX: axotomy; AX+FS: axotomy followed by nerve coaptation with fibrin sealant derived from snake venom; AX+CFS: axotomy followed by coaptation with commercial fibrin sealant.