Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis

Figure 3

Quantitative analysis of endophilin isoform knockdown on sustained neurotransmitter release. (a) Representative traces of excitatory postsynaptic currents (EPSCs) in nontransfected neurons evoked by 100 pulses at 5 Hz in neurons transfected with control or endophilin siRNA. (b–d) Changes in short-term synaptic depression (STD) in nontransfected neurons evoked by stimulation of 100 pulses at 5 Hz in neurons transfected with endophilin siRNA ((b), Endo1 siRNA, ; (c), Endo2 siRNA, ; and (d), Endo3 siRNA, ). (e) Average normalized amplitude of the last 20 responses in endophilin siRNA-transfected neurons compared with that in scrambled negative control- (NC-) transfected neurons; . (f–h) Normalized amplitude of EPSCs evoked by multiple train stimulations in neurons transfected with NC or endophilin siRNA. (f), Endo1 siRNA, ; (g), Endo2 siRNA, ; and (h), Endo3 siRNA, . , .

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