Blockade of NR2A-Containing NMDA Receptors Induces Tau Phosphorylation in Rat Hippocampal Slices

Figure 4

NR2B-containing receptors play a role in Tau phosphorylation induced by NVP. Phosphorylated Tau levels at Ser199 were estimated by Western blotting on cell extracts obtained from acute hippocampal slices treated with 50 nM NVP for 2 hours alone or in combination with 10 $\mu$M NBQX and 10 $\mu$M RO25-6981. The data, expressed relative to total Tau (i.e., Tau-5) levels, are means ± SEM of 3 measurements per cell extract obtained from 4 different rats. Statistical analysis was performed by one-way ANOVA followed by Neuman-Keuls’ post hoc test. ${}^{*}P<.05\text{,}$${}^{***}P<.001$, drug-treated versus control.