Transcription Factor EB Is Selectively Reduced in the Nuclear Fractions of Alzheimer’s and Amyotrophic Lateral Sclerosis Brains

Figure 3

Reduced expression of TFEB protein in ALS nuclear fractions. Lysates of patients brains diagnosed with ALS and NC were prepared, and nuclear and cytosolic fractions were separated as in Figures 1 and 2. Actin and lamin were detected as loading controls and used for normalization of TFEB protein levels. Quantitation by ImageJ revealed significantly reduced (62%) normalized (to lamin) levels of TFEB protein in the nuclear fractions, but no change in the cytosolic fractions. The data are mean ± SEM. by Student’s paired -test, per group.