Recombinant Erythropoietin Provides Protection against Renal Fibrosis in Adenine-Induced Chronic Kidney Disease

Figure 4

rEPO increased EPOR and βcR protein expression and induced coexpression in Ad-CKD rats. (a) Representative western blotting image of EPOR and βcR and β-tubulin. (b) Relative expression of EPOR and βcR. Significant difference in the Ad-CKD+rEPO group (). (c) Immunoprecipitation, cellular lysates were incubated with anti-βcR Ab overnight at 4°C in gentle shaking (1 μg IgG per 100 μg in 1 mL cell lysate) then immunoprecipitated (IP) with 20 μL of A/G-plus agarose per mL and immunoblotted with anti-EPOR.

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