Nicotine Synergizes with High-Fat Diet to Induce an Anti-Inflammatory Microenvironment to Promote Breast Tumor Growth

Figure 4

Quantitative gene expression analysis of HCC70 cells following ex vivo incubation with subcutaneous adipose tissue (SAT) from nude mice and ±NIC. HCC70 and HCC1806 breast cancer cells (5×10⁶ cells) preincubated with SAT ± NIC (1 μM) for 48 h, following which (a) quantitative gene expression analysis of MCSCs was performed using human-specific primer sets: HCC70 (left panel) and HCC1806 (right panel). (b) FACS analysis showing relative changes in OCT4 population in HCC70 cells incubated with SAT and ±NIC. Representative FACS analysis (left panel) and quantitative presentation () of OCT4 (right panel) is shown. (c) FACS analysis showing relative changes in SOX2 population in HCC1806 cells incubated with SAT and ±NIC. Representative FACS analysis (left panel) and quantitative presentation () of SOX2 (right panel) is shown. (d, e) Quantitative gene expression analysis of HCC70 or HCC1806 with SAT + NIC for beige-adipocyte (CD137 and TMEM26), anti-inflammatory cytokines (IL13, 1L10, and IL4) and tissue-resident macrophages (CD163) using species-specific primer sets. (d) HCC70 + SAT + NIC: human (left panel) and mouse (right panel). (e) HCC1806 + SAT + NIC: human (left panel) and mouse (right panel). and().

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