Negligible Effect of Estrogen Deficiency on Development of Skeletal Changes Induced by Type 1 Diabetes in Experimental Rat Models

Table 4

Effects of diabetes (DM) and/or estrogen deficiency on the histomorphometric parameters of compact bone of the femoral diaphysis in rats.

Parameter/group		Nonovariectomized (NOVX) rats		Ovariectomized (OVX) rats		Two-way ANOVA
		Control	DM	Control	DM	OVX	DM	OVXx DM
Diaphysis	Tt.Ar (mm^2)				^XX	NS		NS
	Ct.Ar (mm^2)				^XXX	NS		NS
	Ma.Ar (mm^2)					NS	NS	NS
	Ma.Ar/Tt.Ar					NS	NS	NS

Bilateral ovariectomy was performed 5 weeks before the end of the experiment. Diabetes was induced 3 days after the ovariectomy by a single administration of streptozotocin (60 mg/kg i.p.). Results are presented as (). Two-way ANOVA followed by Fisher’s LSD test was used for evaluation of the significance of the results. , —significantly different from the NOVX control rats; ^XX, ^XXX—significantly different from the OVX control rats; Tt.Ar: transverse cross-section area of the whole diaphysis; Ct.Ar: transverse cross-section area of the cortical bone; Ma.Ar: transverse cross-section area of the marrow cavity; Ma.Ar/Tt.Ar: transverse cross-section area of the marrow cavity/diaphysis ratio.