C1q/TNF-Related Protein 9 Inhibits Coxsackievirus B3-Induced Injury in Cardiomyocytes through NF-κB and TGF-β1/Smad2/3 by Modulating THBS1

Figure 4

Inhibition of THBS1 protected H9c2 cells against CVB3-induced apoptosis, inflammation, and fibrosis. (a, b) H9c2 cells were transfected with the small interfering RNAs against THBS1 (si-THBS1) or its control vectors (si-NC) for 48 h before infected with CVB3. Then, the expression of THBS1 mRNA and protein of H9c2 cells was detected. (c) The cell viability and (d) the apoptosis rate were detected after si-THBS1 transfection. (e, f) The protein expression of Bcl-2, Bax, and cleaved caspase 3 of CVB3-induced H9c2 cells was determined after si-THBS1 transfection. (f, g) The mRNA expression and the secretion levels of TNF-α, IL-6, and IL-1β were measured after si-THBS1 transfection. (h, i) After si-THBS1 transfection, the mRNA and protein expression of fibronectin, collagen I, and collagen III was detected. vs. the CVB3 group. in each group.

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