C1q/TNF-Related Protein 9 Inhibits Coxsackievirus B3-Induced Injury in Cardiomyocytes through NF-κB and TGF-β1/Smad2/3 by Modulating THBS1

Figure 2

CTRP9 protected H9c2 cells against CVB3-induced apoptosis, inflammation, and fibrosis. (a, b) H9c2 cells were transfected with recombinant adenovirus vectors containing full-length rat CTRP9 (Ad-CTRP9) or its control vectors (Ad-NC) for 48 h before infected with CVB3. Then, the expression of CTRP9 mRNA and protein of H9c2 cells was measured. (c) The cell viability and (d) the apoptosis rate were detected after Ad-CTRP9 transfection. (e, f) The protein expression of apoptosis-related factors, including Bcl-2, Bax, and cleaved caspase 3, was measured after Ad-CTRP9 transfection. (f, g) The mRNA expression and secretion levels of proinflammatory factors TNF-α, IL-6, and IL-1β were measured after Ad-CTRP9 transfection. (h, i) The mRNA and protein expression of pro-fibrosis-related genes fibronectin, collagen I, and collagen III was detected after Ad-CTRP9 transfection. vs. the CVB3 group. in each group. Bcl-2: B-cell lymphoma-2; Bax: B-cell lymphoma-2-associated X; TNF-α: tumor necrosis factor α; IL-6: interleukin 6; IL-1β: interleukin 1β.

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