Research Article
Enhanced Activity by NKCC1 and Slc26a6 Mediates Acidic pH and Cl− Movement after Cardioplegia-Induced Arrest of db/db Diabetic Heart
Figure 5
Supportive function of CAIV on SLC26A6 activity and enhanced CBE activity in db/db cardiac strips. (a) CBE activity was assessed by measuring changes in pHi in LV cardiac strips of WT (open black square) and db/db (closed red square) mice following cardioplegia-induced arrest. Full image of CBE activity was represented in the square box, and magnified images were obtained from the dotted line. (b) Analysis of CBE activity. The slope of pHi was measured as CBE activity in 0 Cl−. The bars show the (, ). (c) Changes in pHi by CBE activity of human SLC26A6- (A6-) overexpressed HEK293T cells with and without human CA IV. (d) Analysis of CBE activity. The slope of pHi was measured in CBE activity in the 0 Cl−. The bars represent the (, ). (e) Changes in pHi by CBE activity with (closed blue square) and without (closed black square) 50 nM PMA and (f) analysis of CBE activity. The bars represent the (, ). (g) Analysis of Cl− transporting activity with MQAE technique between WT and db/db LV strips. The bars represent (, ).
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