Enhanced Activity by NKCC1 and Slc26a6 Mediates Acidic pH and Cl⁻ Movement after Cardioplegia-Induced Arrest of db/db Diabetic Heart

Figure 2

Enhanced NKCC1 protein in db/db cardiac tissues. (a) Serum glucose concentration in the WT () and db/db () mice (). Dotted line represents glucose 250 mg/dL. (b) Expression of phosphoNKCC1 (pNKCC1) and NKCC1 protein level in LV and RV cardiac strips of WT and db/db mice following cardioplegia-induced arrest. The β-actin was used as the loading control. (c) Analysis of band intensity of NKCC1 and pNKCC1 in LV. The bars show the (, ). Immunolocalization of NKCC1 (red) and DAPI (blue) in LV of WT (d) and db/db mice (e). Right images of (d, e) are magnified. The scale bars represent 20 μm. (f) Analysis of intensity of NKCC1 between WT and db/db LVs. The bars show the (, ).

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