Interleukin-7 Resensitizes Non-Small-Cell Lung Cancer to Cisplatin via Inhibition of ABCG2

Figure 6

Both IL-7R knockdown and PI3K activation inhibited the synergetic effect by IL-7 on decreased expression of ABCG2 by DDP. After being pretreated with si IL-7R, 740 Y-P, or control, the A549/DDR cells were exposed to IL-7 (2 ng/mL) and DDP (0.5 μg/mL). At 48 h, the cells were harvested for examinations on the expressions of p-AKT and ABCG2 by immunofluorescence (a) and with Western blot, along with PI3K, p-PI3K, and IL-7R (b, c). NC means cells were pretreated with negative control siRNA. , as compared with the IL-7+DDP+NC group; , as compared with the IL-7+DDP+si IL-7R group. Data are presented in terms of .

(a)

(b)

(c)