The Effect and Mechanism of TLR9/KLF4 in FFA-Induced Adipocyte Inflammation

Figure 4

Detection of TLR9 regulation on KLF4 and key factors. (a) TLR9 mRNA expression level in MOI = 0.3, 3, and 10 and time = 72, 96, and 120 h, respectively; (b) oil red O staining results of the mock, negative control, and virus infection groups; (c, d) upregulated TLR9, detecting theTLR9 and KLF4 mRNA and protein expression levels; (e) upregulated TLR9, detecting the mRNA expression level of P65, IL-6, MCP-1, IL-1β, and APN; (f) the fluorescence in concentration = 10, 50, and 100 nmol/L and time = 24 h and 48 h; (g) the interference efficiency of three different TLR9-siRNA; (h) oil red O staining results of the mock, negative control, and TLR9-siRNA groups; (i, j) downregulated TLR9, detecting the mRNA and protein expression levels of TLR9 and KLF4. -test, , ; the difference has a statistical significance; (k) overexpressing KLF4 while knocking down TLR9, detecting the mRNA expression level of IL-6, MCP-1, TNF-α, and APN. ANOVA test, , compared with si-NC; , compared with si-TLR9.

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