Epithelial Cells Attenuate Toll-Like Receptor-Mediated Inflammatory Responses in Monocyte-Derived Macrophage-Like Cells to Mycobacterium tuberculosis by Modulating the PI3K/Akt/mTOR Signaling Pathway

Table 1

The real-time qRT-PCR primers used in this study.

Primers	GenBank number	Sequence (5-3)	Tm	Size (bp)
β-Actin	NM_001101.3	F: TTCGTGGATGCCACAGGACT	61°C	212
		R: GGGAAATCGTGCGTGACATT
IL-1β	NM_000576.2	F: ATGACCTGAGCACCTTCTTTC	62°C	82
		R: TGCACATAAGCCTCGTTATCC
IL-2	NM_000586.3	F: CTCACCAGGATGCTCACATTTA	60°C	240
		R: TACAATGGTTGCTGTCTCATCA
IL-6	NM_000600.3	F: GACAGCCACTCACCTCTTCAG	59°C	172
		R: CATCCATCTTTTTCAGCCATC
IL-8	NM_000584.3	F: TTGCCAAGGAGTGCTAAAGAA	61°C	215
		R: GCCCTCTTCAAAAACTTCTCC
IL-10	NM_000572.2	F: TTTAAGGGTTACCTGGGTTGC	60°C	98
		R: TTGATGTCTGGGTCTTGGTTC
IL-12β	NM_000882.3	F: AGCAGTGAGGTCTTAGGCTCTG	60°C	179
		R: TTGGGTTCTTTCTGGTCCTTTA
TNF-α	NM_000594.2	F: TAGCCCATGTTGTAGCAAACC	63°C	136
		R: ATGAGGTACAGGCCCTCTGAT
MyD88	NM_002468.4	F: CCAGTTTGTGCAGGAGATGA	61°C	288
		R: AGGATGCTGGGGAACTCTTT
TLR2	NM_003264.3	F: ATGCTGCCATTCTCATTCTTCT	60°C	101
		R: CTCCAGGTAGGTCTTGGTGTTC
TLR4	NM_138554.3	F: CTTCTCAACCAAGAACCTGGAC	60°C	158
		R: TAGAGAGGTGGCTTAGGCTCTG
TLR6	NM_006068.4	F: CTACCGCTGAAAACCAAAGTCT	60°C	322
		R: ACTCACAATAGGATGGCAGGAT
TLR8	NM_138636.4	F: CCTGGCTCACCATTTGTTTTA	60°C	198
		R: TTTTTGTCTCGGCTCTCTTCA
NF-κB	NM_002502.5	F: AGGAGAGGATGAAGGAGTTGTG	62°C	218
		R: CCAGAGTAGCCCAGTTTTGTC
TRAF6	NM_145803.2	F: TAGCCCTGGATTCTACACTGG	63°C	215
		R: CTTCGTGGTTTTGCCTTACAG