Comparative Expression Analyses of Pro- versus Anti-Inflammatory Mediators within Synovium of Patients with Joint Trauma, Osteoarthritis, and Rheumatoid Arthritis

Figure 5

Detection of mRNA (a) and the number of positive cells of inflammatory (IL-1β, TNF-α, and 5-LOX) versus anti-inflammatory (15-LOX, FPR2, and IL-10) mediators (b and c) in patients with joint trauma (JT). (a) Quantification of mRNA of pro- versus anti-inflammatory mediators shows that there is a balance between expressions of pro- versus anti-inflammatory mediators in JT synovium. (b) Quantitative analysis of immunofluorescence microscopy of pro- versus anti-inflammatory mediators shows that the number of cells expressing anti-inflammatory mediators was comparable with those containing proinflammatory mediators in JT synovium. Data are shown as means ± SEM. ^∗Relative to control (, one-way ANOVA followed by Tukey’s test). (c) Confocal microscopy of pro- (red fluorescence; (a), (b)) and anti-inflammatory mediators (green fluorescence; (c), (d)) double immunofluorescence (e–h) in JT synovium. Note that anti-inflammatory mediator expression was comparable with those containing inflammatory mediators in JT synovium. Bar = 40 μm.

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