IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex

Figure 1

Reduction of γc surface expression with IL-7. (a) Time dependent kinetics of γc expression with IL-7. T cells were stimulated in the presence or absence of IL-7, and the cytokine receptor expression was analyzed by FACS at the indicated time points. The maximum value of -axis is 250 for 10-minute to 4-hour histograms and is 200 for 16-hour histogram. Histograms are representative of six independent experiments. (b, c) Summary of flow cytometry analysis for γc (4G3) and IL-7Rα expression kinetics in the presence of IL-7. Data are summary of six independent experiments (mean and SEM). (d) The early time point kinetics of γc (4G3) expression in the presence of IL-7. Data are summary of three independent experiments (mean and SEM). (e) Kinetics of γc (4G3) surface expression on T cells under different concentration of IL-7. T cells were stimulated with an indicated concentration of IL-7 for 10 minutes, and the surface γc expression was analyzed by FACS. Data are summary of three independent experiments (mean and SEM). (f) Freshly isolated LNT cells from WT mice were stimulated with IL-7 for the indicated time and assayed for intracellular pSTAT5 content. Fold induction of pSTAT5 content was calculated to that of fresh LNT cells, set as 1. Data are summary of two independent experiments (mean and SEM).

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