HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes

Figure 4

Bioplex multiplex system analysis of supernatants from HaCaT cells stimulated by TNFα during cell differentiation. The amount of chemokines (a), cytokines, and growth factors (b) was measured by Multiplex system technology in the supernatants of HaCaT cells plated at 1.0 × 10⁴ cells/cm², grown in low (a) and high (c) Ca²⁺-containing medium for 6 (A6 and C6) and 14 (A14 and C14) days (white bars), and treated with 10 ng/ml TNFα for 48 hours (black bars) in a serum-free medium. The data are expressed as pg/10⁶ cells.

(a)

(b)