HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes

Figure 3

In vitro release of CXCL8/IL8, VEGF, and MMP-9 from HaCaT cells stimulated by TNFα during cell differentiation. The amount of CXCL8/IL8, VEGF, and MMP-9 was measured by ELISA in the supernatants of HaCaT cells plated at the same density (1.0 × 10⁴ cells/cm²), grown in low (a) and high (C) Ca²⁺-containing medium for 6 (A6 and C6) or 14 (A14 and C14) days (white bars), and treated with 10 ng/ml TNFα for 6 or 24 hours (black bars), as indicated, in the absence (a) or the presence (b) of serum. The data are expressed as pg/10⁶ cells, and values are the mean ± SD of at least three independent experiments in duplicate.

(a)

(b)