Distinct Signature of Oxylipid Mediators of Inflammation during Infection and Asymptomatic Colonization by E. coli in the Urinary Bladder

Figure 5

Fatty acid content of the urinary bladder is increased during acute UTI. Fatty acid content of urinary bladders collected from UPEC-infected and ABEC-colonized mice (female CBA/J, ) was determined by LC-MS/MS. Infection with UPEC strain CFT073 results in significantly higher levels of linoleic acid (a), arachidonic acid (b), eicosapentaenoic acid (c), and docosahexaenoic acid (d) in the bladder compared to PBS controls and ABEC-colonized mice during the onset of UTI (6 h PI). Bladder fatty acid levels at 48 h PI are not significantly different between groups. Mean + SEM are presented here. , , and , ANOVA with Bonferroni’s multiple comparison test. PBS, sham-inoculated control; UPEC, uropathogenic E. coli; ABEC, asymptomatic bacteriuric E. coli; CFT, UPEC strain CFT073; ABU, ABEC strain 83972; h PI, hour postinoculation.

(a)

(b)

(c)

(d)