TY - JOUR A2 - Prattichizzo, Francesco AU - Laura, Costarelli AU - Robertina, Giacconi AU - Piacenza, Francesco AU - Basso, Andrea AU - Pacetti, Deborah AU - Balzano, Michele AU - Gagliardi, Riccardo AU - Frega, Natale Giuseppe AU - Mocchegiani, Eugenio AU - Provinciali, Mauro AU - Malavolta, Marco PY - 2017 DA - 2017/12/12 TI - Gene Expression, Oxidative Stress, and Senescence of Primary Coronary Endothelial Cells Exposed to Postprandial Serum of Healthy Adult and Elderly Volunteers after Oven-Cooked Meat Meals SP - 3868545 VL - 2017 AB - Epidemiological studies have linked high consumption of meat with major age-related diseases including cardiovascular diseases. Abnormal postprandial increases in plasma lipids after a meat meal have been hypothesized among the pathogenetic mechanisms. However, it is still unknown if the postprandial serum derived after a normal meat meal is able to affect endothelial function, and if the type of meat and the age of the donors are critical factors. Here, we show the effects of postprandial sera derived from healthy adults and elderly volunteers who consumed meat meals on human coronary artery endothelial cell (HCAEC) oxidative stress, gene expression, DNA damage, and cellular senescence. We observed that a single exposure to postprandial serum induces a slight increase in ROS that is associated with modulation of gene expression pathways related to oxidative stress response and metabolism. The postprandial-induced increase in ROS is not associated with a measurable DNA oxidative damage. However, repeated exposure to postprandial serum induces an acceleration of cellular senescence. Taking into account the deleterious role of cellular senescence in age-related vascular diseases, the results suggest a new mechanism by which excessive meat consumption and time spent in postprandial state may affect health status during aging. SN - 0962-9351 UR - https://doi.org/10.1155/2017/3868545 DO - 10.1155/2017/3868545 JF - Mediators of Inflammation PB - Hindawi KW - ER -